Architecture and active site of particulate methane monooxygenase

Redox potentiometry studies of particulate methane monooxygenase: support for a trinuclear copper cluster active site.

Particulate methane monooxygenase genes in methanotrophs.

A 45-kDa membrane polypeptide that is associated with activity of the particulate methane monooxygenase (pMMO) has been purified from three methanotrophic bacteria, and the N-terminal amino acid sequence was found to be identical in 17 of 20 positions for all three polypeptides and identical in 14 of 20 positions for the N terminus of AmoB, the 43-kDa subunit of ammonia monooxygenase. DNA from ...

Methane and Trichloroethylene Degradation by Methylosinus trichosporium OB3b Expressing Particulate Methane Monooxygenase.

Whole-cell assays of methane and trichloroethylene (TCE) consumption have been performed on Methylosinus trichosporium OB3b expressing particulate methane monooxygenase (pMMO). From these assays it is apparent that varying the growth concentration of copper causes a change in the kinetics of methane and TCE degradation. For M. trichosporium OB3b, increasing the copper growth concentration from ...

Biomimetic Modeling of the Active Site of Soluble Methane Monooxygenase Hydroxylase (sMMOH)

ing the hydrogen atom via a radical mechanism. When H3C radical recombines with the OH/H2O ligand at the diiron core, methanol is extruded from the active site. Efficient catalysis is made possible by the regulatory protein MMOB. 33 Scheme 1. Proposed catalytic cycle of sMMOH, showing the diiron core transformation. 33 Biomimetic Modeling of the Active Site of sMMOH All the knowledge accumulat...

Comparative analysis of the conventional and novel pmo (particulate methane monooxygenase) operons from methylocystis strain SC2.

In addition to the conventional pmoA gene (pmoA1) encoding the active site polypeptide of particulate methane monooxygenase, a novel pmoA gene copy (pmoA2) is widely distributed among type II methanotrophs (methane-oxidizing bacteria [MOB]) (M. Tchawa Yimga, P. F. Dunfield, P. Ricke, J. Heyer, and W. Liesack, Appl. Environ. Microbiol. 69:5593-5602, 2003). Here we report that the pmoA1 and pmoA2...